Use of thiolated oligonucleotides as anti-fouling diluents in electrochemical peptide-based sensors.
نویسندگان
چکیده
We incorporated short thiolated oligonucleotides as passivating diluents in the fabrication of electrochemical peptide-based (E-PB) sensors, with the goal of creating a negatively charged layer capable of resisting non-specific adsorption of matrix contaminants. The E-PB HIV sensors fabricated using these diluents were found to be more specific and selective, while retaining attributes similar to the sensor fabricated without these diluents. Overall, these results highlight the advantages of using oligonucleotides as anti-fouling diluents in self-assembled monolayer-based sensors.
منابع مشابه
High performance liquid chromatographic analysis of reduction products of a thiolated DNA for immobilization on gold nanoparticles
DNA-based nano-biosensors are emerging scope in the field of biosensors. Many synthetic single stranded functional DNAs have been applied for development of such sensors, recently. Immobilization of DNA oligonucleotides on the surface of gold nanoparticles is a key step in the development of most colorimetric nano-biosensors. The bound DNA is usually thiolated and forms Au-S covalent bond to th...
متن کاملApplication of Graphene and Graphene Oxide for modification of electrochemical sensors and biosensors: A review
This paper gives a comprehensive review about the most recent progress in graphene and graphene oxide based electrochemical sensors and biosensors. Graphene, emerging as a true 2-dimensional material, has received increasing attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization and mass product...
متن کاملApplication of Graphene and Graphene Oxide for modification of electrochemical sensors and biosensors: A review
This paper gives a comprehensive review about the most recent progress in graphene and graphene oxide based electrochemical sensors and biosensors. Graphene, emerging as a true 2-dimensional material, has received increasing attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization and mass product...
متن کاملOptimisation and Characterisation of Anti-Fouling Ternary SAM Layers for Impedance-Based Aptasensors
An aptasensor with enhanced anti-fouling properties has been developed. As a case study, the aptasensor was designed with specificity for human thrombin. The sensing platform was developed on screen printed electrodes and is composed of a self-assembled monolayer made from a ternary mixture of 15-base thiolated DNA aptamers specific for human thrombin co-immobilised with 1,6-hexanedithiol (HDT)...
متن کاملElectrochemical Characterization of Protein Adsorption onto YNGRT-Au and VLGXE-Au Surfaces
The adsorption of the proteins CD13, mucin and bovine serum albumin on VLGXE-Au and YNGRT-Au interfaces was monitored by electrochemical impedance spectroscopy in the presence of [Fe(CN)6](3-/4-). The hydrophobicity of the Au surface was tailored using specific peptides, blocking agents and diluents. The combination of blocking agents (ethanolamine or n-butylamine) and diluents (hexanethiol or ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 50 36 شماره
صفحات -
تاریخ انتشار 2014